Novel coronavirus (2019-nCoV) antigen detection technology and solution
Time: Jan 12, 2022

COVID-19 There have been more than 300 million confirmed cases of new coronary pneumonia worldwide, posing a huge threat to global public health security. In order to meet the requirements for rapid testing of 2019-nCoV, the US FDA issued the first Emergency Use Authorization (EUA) notification for the 2019-nCoV antigen test on May 9, 2020. The 2019-nCoV antigen test is a novel test that rapidly detects unique protein fragments on or within the virus by using a swab to collect a sample from the mouth or nose.

JINYE has various types of antigen detection kits for novel coronavirus with high sensitivity and quick results.

The significance of novel coronavirus antigen detection

Novel coronavirus antigen detection can directly detect whether human samples contain novel coronavirus, the diagnosis is rapid and accurate, and the requirements for equipment and personnel are low. The double-antibody sandwich method uses two antigen-specific antibodies to identify and bind to a target. Different epitopes of the antigen can greatly reduce the probability of cross-reaction, thereby effectively improving its specificity. The cost of antigen detection is lower, it is easy to produce, and the detection speed is very fast, which is suitable for a large-scale new coronavirus detection.

The principle of novel coronavirus antigen detection

The new coronavirus antigen detection method uses colloidal gold. The N protein of the new coronavirus can be used as an immunogen to stimulate plasma cells to produce specific antibodies after the virus infects the human body. According to the principle of double-antibody sandwich ELISA, the sample is dropped on the sample pad, and then passes through the binding pad, the detection line (T line) and the quality control line (C line) on the NC membrane in turn by liquid chromatography. The binding pad contains labeled antigen-specific antibodies that can bind to the antigen (viral protein) in the sample. When the liquid flow reaches the detection line (T line), the second antigen-specific antibody immobilized on this line Binding to the antigen again will give a positive result. The quality control line (C line) is coated with IgY antibody, which can bind to the antibody in the sample pad and is used to judge whether the chromatography process is smooth.

The above reagent kits are available from JINYE. Can be exported to various countries. You can consult us for details.

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