Introduction of Novel Coronavirus Antigen Detection
Time: Jan 12, 2022

Novel coronavirus antigen detection can directly detect whether human samples contain novel coronavirus, the diagnosis is rapid and accurate, and the requirements for equipment and personnel are low. The double-antibody sandwich method uses two antigen-specific antibodies to identify and bind to a target. Different epitopes of the antigen can greatly reduce the probability of cross-reaction, thereby effectively improving its specificity.

1. What is the principle of antigen detection kit?

Antigens such as N protein, E protein and S protein of the COVID-19 can be used as immunogens to stimulate plasma cells to produce specific antibodies after the virus infects the human body. According to the principle of double-antibody sandwich ELISA, the sample is dropped on the sample pad, and then passes through the binding pad, the detection line (T line) and the quality control line (C line) on the NC membrane in turn by liquid chromatography. The binding pad contains labeled antigen-specific antibodies that can bind to the antigen (viral protein) in the sample. When the liquid flow reaches the detection line (T line), the second antigen-specific antibody immobilized on this line Binding to the antigen again will give a positive result. The quality control line (C line) is coated with IgG antibody, which can bind to the antibody in the sample pad to judge whether the chromatography process is smooth.

2. What are the types of antigen test samples?

Usually samples from infected sites, such as oropharyngeal swabs, nasopharyngeal swabs, sputum, serum, plasma, etc.

3. Four steps to detect the COVID-19

1. Drop the sample processing liquid into the sample processing tube;

2. Stir the sampling swab and squeeze the tube wall, so that the sample is fully eluted into the treatment solution;

3. Take out the test card and add sample to the round hole;

4. Wait 15 minutes to read the results.

4. What are the problems with antigen detection?

■ False negative, antigen detection requires higher sensitivity. Since the new coronavirus mainly invades the lower respiratory tract such as the alveoli, sampling from the upper respiratory tract such as the nasopharynx and oropharynx may not be able to obtain pathogens, or the amount of virus contained in the sample is relatively high. These factors can cause missed detection;

JINYE has a variety of COVID-19 rapid test kits that can be used for early screening of infection, which is an effective complement to nucleic acid (PCR) testing. You can do it at home and get results in 15 minutes.

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