COVID-19 reagent product introduction
Time: Jan 05, 2022

The PCR method refers to the polymerase chain reaction, which can greatly increase the trace amount of DNA. When used to detect the COVID-19 virus, since the COVID-19 virus is an RNA virus, it is necessary to reverse transcribe the viral RNA into DNA before performing PCR detection.

The principle of fluorescent PCR detection is that with the progress of PCR, the reaction products continue to accumulate, and the intensity of the fluorescent signal also increases in proportion. Finally, the change in the amount of product is monitored by the change in fluorescence intensity, thereby obtaining a fluorescence amplification curve.

This is currently the most commonly used method for COVID-19 virus nucleic acid detection. However, the following factors may cause errors in the test results:

Improper storage or failure to submit for testing: RNA viruses are easily degraded. Therefore, after obtaining patient samples, they need to be stored in a standardized manner and tested as soon as possible. Otherwise, the test result is likely to be inaccurate.

Error interpretation of results: The currently approved testing products are all selected based on the open reading frame 1a/b, envelope protein and nucleocapsid protein of the novel coronavirus genome. However, the design of detection primers and probes for different products are different, and there are differences in detection and interpretation of single-target segments, dual-target segments, and three-target segments. Therefore, if the interpretation is not strictly in accordance with the different instructions, it may lead to wrong judgment of the result.

Novel coronavirus IgM antibody detection kit (fluorescence immunochromatography)

The principle of the new coronavirus IgM antibody detection kit (fluorescence immunochromatography) is based on dry fluorescent labeling technology and immunochromatography technology. It uses whole blood, serum or plasma as samples, and is equipped with a fully automatic fluorescence immunoassay. Fully automatic puncture and sample introduction, realize "sample in, result out", avoid the generation of aerosol to the greatest extent, and reduce the risk of pollution to the operator and the environment.

It is a new membrane detection technology based on the specific immune response of antigen and antibody.

The kit contains a test card, the test card includes a sample pad, a fluorescent pad, a detection pad, a suction pad and a bottom plate, the fluorescent pad is coated with a coating of fluorescent microsphere-labeled antibodies, and the antibodies include recombinant SARS-CoV-2 antigen.

Novel coronavirus (2019-nCoV) IgM antibody detection kit (colloidal gold)

The detection principle of the new coronavirus (2019-nCoV) IgM antibody detection kit (colloidal gold method) is that the detection principle of the colloidal gold-labeled antibody IgM, and the new coronavirus antigen is coated on the nitrocellulose membrane, using the indirect method of colloidal gold immunochromatography The principle is to achieve the purpose of detecting the new coronavirus IgM antibody in human serum.

JINYE has a professional export qualification for COVID-19 virus reagents, and has various types of COVID-19 reagents. You can get your test results quickly, professionally and accurately.

Novel coronavirus IgM antibody detection kit (enzyme-linked immunoassay)

Enzyme-linked immunoassay kit for novel coronavirus IgM antibody and its preparation method, involving the field of biomedicine, including enzyme conjugate, sample diluent, negative control solution, positive control solution, substrate A solution, substrate B solution, and concentrated The washing solution, termination solution and the coating plate pre-coated with the COVID-19 virus antigen are tested by the immunospecific reaction of the antigen and antibody to qualitatively determine whether there is a new coronavirus IgM antibody in the test sample, which has high specificity and resistance. The characteristics of strong interference, and the application structure is simple, the detection cost is low, and the accuracy is good. The new type of coronavirus IgM antibody enzyme-linked immunoassay detection method of the present invention is detected by the immunospecific reaction of the antigen and antibody, and has high specificity , Features of strong accuracy, simple steps, reasonable design, suitable for promotion.

Specifically, it is an enzyme-linked immunoassay that uses the principle of indirect method to achieve qualitative detection of new coronavirus IgM antibodies in serum or plasma samples.

Novel coronavirus antibody detection kit (chemiluminescence method)

The principle of the new coronavirus antibody detection kit (chemiluminescence method) is that the recombinant protein of the virus is labeled with high-tech material magnetic beads, which is used to bind the virus antibody IgM in the blood sample, and then use the secondary antibody to identify the antibody. The secondary antibody is coupled with alkaline phosphatase, which will generate a light signal after adding the substrate. The chemiluminescence instrument detects photons through a high-sensitivity PMT (photomultiplier tube) to achieve high-sensitivity detection at the pg (picogram) level.

Characteristics of various detection methods

The colloidal gold method is easy to operate and can be directly visually interpreted. Generally, the test can be completed in about 15 minutes;

Immunofluorescence chromatography is as convenient as the colloidal gold method, and the detection is rapid, but it requires instrument interpretation;

Enzyme-linked immunoassay can be interpreted by a conventional microplate reader. Generally, the sensitivity is high, but the detection time is longer (about 1.5 hours), and there are many operating steps. Measures should be taken to avoid infection during the operation.

The chemiluminescence method is generally more sensitive. The automatic chemiluminescence immunoassay analyzer is used to complete the detection without too much manual operation. The detection time is generally about half an hour.

Scope of application:

It is used for in vitro qualitative detection of new coronavirus (2019-nCoV) IgM/IgG antibodies in human serum, plasma and venous whole blood samples.

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